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Although the regulatory pathways governing lipid A modification are often species specific, the PmrAB and PhoPQ systems are conserved and functionally active in several Gram-negative pathogens, including A

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For cell number measurements in 6-well plates, the medium was aspirated, the wells were rinsed with PBS and the cells were detached by incubating with 500 l of 0.25% trypsin (Thermo Scientific, 25200056) for 20 min at 37 C